Inducible CRISPR activation screen for interferon-stimulated genes identifies OAS1 as a SARS-CoV-2 restriction factor.
Interferons establish an antiviral state through the induction of hundreds of interferon-stimulated genes (ISGs).The mechanisms and viral specificities for most ISGs remain incompletely understood.To enable high-throughput interrogation of ISG antiviral functions in pooled genetic screens while mitigating potentially confounding effects of endogeno